Subsequent physical integration of internalized ssDNA into the recipient chromosome by homologous recombination requires dedicated cytosolic ssDNA–processing proteins.
In many species, transformability is a transient property relying on a specialized membrane-associated machinery for binding exogenous double-stranded DNA and internalization of single-stranded DNA (ssDNA) fragments extracted from exogenous DNA. Natural genetic transformation can compensate for the absence of sexual reproduction in bacteria, allowing genetic diversification by frequent recombination. pneumoniae by increasing the likelihood of multiple transformation events in the same cell. We propose that the evolutionary raison d'être of SsbB and its abundance is maintenance of this reservoir, which contributes to the genetic plasticity of S. SsbBΔ7 fulfils the reservoir function, suggesting that SsbB C-ter is not necessary for processing protein(s) to access stored ssDNA.
pneumoniae (compared to chromosomal transformation), the former supports our previous suggestion that SsbB creates a reservoir of ssDNA, allowing successive recombination cycles. While the latter observation explains a long-standing observation that plasmid transformation is very inefficient in S. We show that SsbB is highly abundant, potentially allowing the binding of ∼1.15 Mb ssDNA (half a genome equivalent) that it participates in the processing of ssDNA into recombinants and that, at high DNA concentration, it is of crucial importance for chromosomal transformation whilst antagonizing plasmid transformation. We provide evidence that SsbB directly protects internalized ssDNA. pneumoniae, the latter constructed because SSBs' acidic tail has emerged as a key site for interactions with partner proteins. Here, we report our investigations involving comparison of a null mutant ( ssbB −) and a C-ter truncation ( ssbBΔ 7) of SsbB of S. In Bacillus subtilis and Streptococcus pneumoniae, an alternative SSB, SsbB, is expressed uniquely during competence for genetic transformation, but its precise role has been disappointingly obscure. Bacteria encode a single-stranded DNA (ssDNA) binding protein (SSB) crucial for genome maintenance.
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